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    This dataset provides understorey herbaceous biomass, ground cover and overstorey woody cover response to different fire regimes over a twenty year period at a grassland and open woodland in the tropical savannas of northern Australia. BOTANAL was used to assess understorey herbaceous biomass. Woody canopy cover was derived from digital analysis of oblique aerial imagery taken from a helicopter at the site in 1995 and again in 2013. Woody cover (tree basal area and canopy cover) was also assessed using a bitterlich gauge on BOTANAL ground based transects in 2009. The data could be used to calibrate models of herbaceous growth and woody cover change in response to long term fire. It may be useful for assessing climate change impacts on aboveground carbon sequestration. The fire regimes tested were of varying frequency (every 2, 4 and 6 years) and season (June vs. October) of fire compared to unburnt controls on woody cover and pasture composition. Sites were open to grazing by cattle.